http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108690841-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2425 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
filingDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108690841-B |
titleOfInvention | Method for modifying beta-amylase by mPEG-Mal5000 |
abstract | The invention discloses a method for modifying beta-amylase by mPEG-Mal5000, wherein the mPEG-Mal5000 is used as a modifier, the optimal modification process parameters are that the addition ratio of the mPEG-Mal5000 is 1:4, the modification reaction temperature is 58 ℃, the modification reaction pH is 6.0, and the reaction time is 10 min. The specific enzyme activity of mPEG-Mal 5000-beta-amylase is (2.08 +/-0.055) ten thousand U/mg, and the enzyme activity is improved by 20.10 percent compared with that of unmodified beta-amylase; post-modification enzyme kinetic parameter K m Reduced by 12.95% and V max 26.87% is improved, and the heat resistance of the mPEG-beta-amylase is obviously higher than that of the beta-amylase at the temperature of 30-70 ℃. The pH stability of mPEG-Mal 5000-beta-amylase is obviously higher than that of beta-amylase at pH 6.5-7.5. |
priorityDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.