http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108645905-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-327
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327
filingDate 2018-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108645905-B
titleOfInvention A method of hydrogen peroxide is detected based on solid nano hole
abstract The present invention relates to a kind of methods based on solid nano hole detection hydrogen peroxide, by beating different nano-pores on 10-100nm ultra-thin silicon nitride film, with a variety of silanization treatments, realize nanoporous surface and inner wall differentiation modification, by silane coupled molecule by peroxidase conjugate in nano-pore inner wall, it constructs controllable, stable, unimolecule hydrogen peroxide sersor part in highly sensitive nano-pore, by the nano-pore ion current signal for parsing enzymatic reaction, the model mechanism and rule of single enzyme molecule enzymatic reaction Km and Kcat in nano-pore, with Real_time quantitative detection hydrogen peroxide.The present invention reaches nanomole or less by the controllable modification of differentiation modification and horseradish peroxidase unimolecule in nano-pore inside and outside nano-pore, the small molecule real-time sensing detection method of the specificity of foundation, precision.
priorityDate 2018-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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