http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108642088-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 |
| filingDate | 2018-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8385f5eece744c627f4c63534a784c5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8abb056848358e0c2ee84b4331391376 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30fb1ebe8df9895237e168f248a21d6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ee9ca1346e95745d26d7e12ad668135 |
| publicationDate | 2018-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108642088-A |
| titleOfInvention | Erythrocyte membrane surface modification gene delivery system, preparation method and application |
| abstract | The invention discloses a red blood cell membrane surface modification gene delivery system and its preparation method and application. Preparation: 1. Take blood, remove plasma and buffy coat to obtain sample 1; wash, add 0.25×PBS buffer solution, place in ice bath, make red blood cells The membrane ruptures and releases heme, centrifuges to collect sample 2; washes and centrifuges to obtain sample 3, adds it to PBS buffer solution, and sonicates to obtain sample 4; extrudes back and forth through the nano-polycarbonate film through a liposome extruder Repeatedly, collecting erythrocyte membrane vesicle fluid as sample 5; ultrasonically obtaining nanometer erythrocyte membrane fluid as sample 6; 2. Preparation of gene complex; 3. Adding sample 6 to the gene complex to obtain erythrocyte membrane surface Modified gene delivery systems. The system of the invention has high transfection efficiency and low cytotoxicity. It has good biocompatibility and is stable in the blood, avoiding its rapid elimination in the body, greatly extending the circulation time in the body, which is beneficial to gene delivery and expression. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111803653-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111803653-A |
| priorityDate | 2018-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.