| abstract |
The invention discloses anti-human PD1 antibody and its applications.The PD1 that the present invention gives expression to recombination using mammalian cell expression system is merged with myeloma cell through Mouse spleen cells as mice immunized with antigen and is obtained hybridoma.Inventor is by carrying out the screening to great amount of samples, hybridoma cell strain is obtained, it secretes the monoclonal antibody specific generated with PD1 specific bindings, has very high affinity to PD1, and the monoclonal antibody can highly desirable block the combination of PD1 and PDL1.The heavy chain of the anti-human PD1 monoclonal antibodies of mouse provided by the invention, light chain variable region, increase the sequence of new anti-human PD1 monoclonal antibodies, enrich the type of antibody, be with a wide range of applications to the treatment of tumour, infectious diseases and autoimmune disease. |