| abstract |
A kind of time-resolved fluoroimmunoassay chromatography card detecting vomitoxin and its acetyl derivatives, belongs to immunochromatography and field of detection of food safety.This chromatography card is made of sample pad, label pad, nitrocellulose filter, absorbent filter and PVC bottom plates.Label pad is fixed with a nanometer vomitoxin monoclonal antibody 1 and antibody 2 for europium fluorescent microsphere label.Antibody 1 has high degree of specificity to vomitoxin and 15 acetyl deoxynivalenols；Antibody 2 has high degree of specificity to vomitoxin and 3 acetyl deoxynivalenols.The present invention is based on antigen-antibody immunology principles to be detected.This chromatography blocks to be combined with portable time resolved fluorometric immunity analysis instrument, and for vomitoxin and its Quantitative detection of acetyl derivatives total amount in Grain and its product, high sensitivity, stability are good；0.168 mg/kg of detection limit, detection range 0.17~5 mg/kg, 10~15min of time, it is easy to operate, it is not required to professional training, is widely used in the needs of various occasions and different levels personnel. |