| abstract |
The invention discloses an endo beta-1, 4-glucanase gene from paenibacillus polymyxa, a preparation method and application of the enzyme, namely, the gene of the endo beta-1, 4-glucanase is cloned to an escherichia coli expression vector by utilizing a technical method of genetic engineering to obtain an escherichia coli recombinant strain capable of heterologously expressing the enzyme, and the endo beta-1, 4-glucanase prepared by heterologously expressing the strain can efficiently degrade konjac polysaccharide (also called konjac glucomannan). The endo beta-1, 4-glucanase provided by the invention can be widely applied to the fields of agriculture, food, feed additives, medicines, glucomannan oligosaccharide preparation and the like. |