http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108611296-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-02 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02 |
filingDate | 2018-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108611296-B |
titleOfInvention | Method for high-throughput screening and separation of bacillus thuringiensis and application thereof |
abstract | The invention belongs to the field of microbiology, and discloses a method for high-throughput screening and separation of Bacillus thuringiensis (Bt for short) and application thereof. According to the method provided by the invention, through continuous three-step selective culture with different strategies, the absolute biomass and relative proportion of Bt cells in a sample are greatly improved, the number of colonies needing to be picked for transfer culture and microscopic examination is reduced, the separation efficiency is greatly improved, and the operation is simpler and more convenient; meanwhile, the required sample amount is reduced, high-throughput operation can be realized, and the method is particularly suitable for separating the Bt strain from a sample with small sample amount or a sample with small Bt biomass. |
priorityDate | 2018-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.