| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6865
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate |
2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-108603193-B |
| titleOfInvention |
Split cycle and TAPE amplification |
| abstract |
Described herein are methods and compositions for the quantitative detection of sequences in nucleic acid samples. The method typically involves distributing a nucleic acid sample into a large number of mixture partitions in discrete reaction chambers. On the other hand, the allocated samples were analyzed in a labeled amplicon primer extension (TAPE) reaction. In the TAPE reaction, a pair of 5'-tailed amplification primers, one 5'-tailed forward primer and one 5'-tailed reverse primer are used to amplify the target sequence. In another aspect, the present invention provides methods for quantifying the frequency of wild-type and mutant target nucleic acid fragments in a nucleic acid sample. |
| priorityDate |
2015-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |