abstract |
The invention discloses a construction method and application of a high-efficiency soybean CRISPR/Cas9 system. The Staphylococcus aureus Cas9 protein is split and expressed. In order to improve the editing efficiency and broad spectrum of soybean genome, a soybean SaCas9 editing system is constructed. The invention transforms a low-efficiency editing system into a high-efficiency editing system, which not only expands the application range of soybean genome editing, but also provides new ideas for solving the problem of crop genome editing efficiency in the future. |