http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108570458-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y603-02002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2018-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108570458-B |
| titleOfInvention | Method for preparing glutathione synthetase system |
| abstract | The invention belongs to the technical field of bioengineering, and particularly relates to a method for preparing a glutathione synthetase system from microbial cells. The preparation method of the glutathione synthetase system comprises the following steps: (1) culturing the microorganism producing glutathione synthetase system, centrifugally collecting cells, and crushing the cells; (2) preparing a polypropylene glycol/sodium lactate aqueous two-phase extraction system solution; (3) separating and purifying the glutathione synthetase system by using a two-aqueous-phase extraction system to obtain sodium lactate solution containing the glutathione synthetase system; (4) filtering and concentrating by adopting an ultrafiltration membrane, freezing and drying trapped fluid, and preparing the glutathione synthetase system product. The polypropylene glycol/sodium lactate double-aqueous phase system has a protection effect on enzyme activity, and is particularly suitable for separation and purification of a glutathione synthetase system; the invention can realize the continuous and large-scale preparation of the glutathione synthetase system, and has the advantages of high enzyme activity recovery rate, high purity, simple and convenient production process and lower production cost of the glutathione synthetase system. |
| priorityDate | 2018-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 107.