http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108546741-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 |
filingDate | 2018-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108546741-B |
titleOfInvention | NASBA-ELISA rapid detection kit and detection method for corn transfer-resistant glufosinate-resistant gene component |
abstract | The invention relates to the technical field of biological detection, in particular to an NASBA-ELISA rapid detection kit for corn transfer-resistant glufosinate-resistant gene components, which is characterized by comprising specific primers, capture probes and detection probes designed according to mRNA sequences of glufosinate-resistant gene bar, an optimized NASBA amplification system and an optimized NASBA product detection system. The method can simply and quickly detect the glufosinate-resistant gene component in the corn, has the advantages of high-efficiency specificity and easy popularization, and is also beneficial to early diagnosis of the glufosinate-resistant gene component bar in the transgenic corn; the detection method comprises the steps of designing and artificially synthesizing a specific primer, a capture probe and a detection probe of an mRNA sequence of the glufosinate-resistant gene bar, determining an NASBA amplification reaction program, an NASBA product detection system and the like, avoids the requirements of special equipment and professionals, and is high in result sensitivity and good in repeatability. |
priorityDate | 2018-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 98.