http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108546683-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0603 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-861 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
| filingDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108546683-B |
| titleOfInvention | Method for efficiently producing transgenic buffalo embryos by adenovirus mediation |
| abstract | The invention relates to the technical field of biology, in particular to a method for efficiently producing transgenic buffalo embryos by adenovirus mediation, which is characterized by comprising the following steps: the method comprises the following specific steps of in-vitro culture of buffalo oocytes; in vitro fertilization; preparing a monolayer co-culture system; culturing the embryo in vitro; packaging an adenovirus vector and amplifying the adenovirus; diluting the adenovirus titer; infecting buffalo granular cells with adenovirus; infecting buffalo embryo with adenovirus to obtain transgenic buffalo embryo mediated by adenovirus. The invention has the advantages that: by adopting the technical scheme of the invention, the transgenic buffalo embryos are infected by the adenovirus in a mediated manner, the cleavage rate, the mulberry cyst rate and the transfection blastocyst rate of the buffalo embryos are all obviously improved, and the transfection efficiency can be greatly improved. |
| priorityDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.