http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108531476-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2018-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108531476-B |
| titleOfInvention | Method for concentrating plasmid by freeze drying |
| abstract | The invention discloses a method for concentrating plasmids by freeze drying, which comprises the following steps: extracting plasmids according to the requirements of the small quality-improved particle kit, and finally using100μLddH 2 Elution of O into a 1.5mL EP tube; measuring the plasmid concentration using a ultramicro spectrophotometer NANO DROP; opening the cover of the measured plasmid, sealing a layer of sealing film, and freeze-drying in a vacuum freeze-drying machine; quantitative ddH for the same plasmid 2 Re-dissolving O to ensure that the double distilled water fully contacts the film formed by the dried plasmid liquid and completely dissolves the film; measuring the plasmid concentration with a ultramicro spectrophotometer NANO DROP; comparing the change of plasmid concentration and mass before and after freeze drying, collating data, and counting the change rule of plasmid concentration and volume before and after. The method of the invention has strong stability, and the method is also suitable for concentration of other nucleic acid products with lower concentration. |
| priorityDate | 2018-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419591920 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID9324 |
Total number of triples: 12.