| abstract |
The invention relates to a tissue engineering skin containing a blood vessel and hair follicle structure based on 3D printing and a preparation method thereof, wherein the tissue engineering skin consists of an epidermal layer, an acellular dermal scaffold and a dermal layer, the epidermal layer takes epidermal stem cells as seed cells, is compounded with carrier hydrogel and then is printed on the upper surface of the acellular dermal scaffold by a 3D printer to be differentiated into a normal epidermal structure, the dermal layer takes mesenchymal stem cells, vascular endothelial cells, hair papilla cells and adipose stem cells as seed cells and is compounded with the carrier hydrogel, gelatin sustained-release microspheres compounded with cell factors are printed on the lower surface of the acellular dermal scaffold by the 3D printer, and meanwhile, the hydrogel compound of the seed cells is printed in the gelatin sustained-release microspheres to form a dermal structure with a three-dimensional space structure. |