http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108522281-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2018-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108522281-B
titleOfInvention Breeding method for in vitro mutagenesis of pleione bulbocodioides by utilizing ethyl methanesulfonate
abstract The invention discloses a breeding method for in vitro mutagenesis of pleione bulbocodioides by utilizing ethyl methanesulfonate. The invention provides a method for obtaining a mutant plant of pleione bulbocodioides, which comprises the following steps: callus derived from pleione bulbocodioides was mutagenized with ethyl methanesulfonate. The method for obtaining the mutant plant of the pleione bulbocodioides comprises the following steps: and culturing the mutagenized callus as a regeneration plant. The method for obtaining the mutant plant of the pleione bulbocodioides comprises the following steps: and (4) screening mutant plants from the regenerated plants. The method provided by the invention combines the mutagenesis technology with in vitro culture, and utilizes ethyl methane sulfonate to mutagenize the callus of the pleione bulbocodioides in vitro, so that a large and homogeneous mutagenized population can be obtained. The method greatly improves mutation frequency and breeding efficiency, is beneficial to improving the mutation breeding of the pleione bulbocodioides, promotes the industrial development of the pleione bulbocodioides and has great application and popularization values.
priorityDate 2018-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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