http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108504747-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1cc6d033a98a42862d8bdd40bc1f409b |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
filingDate | 2018-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_942eb0a19a4ca5d50291d6dab28906e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_043e8b340f1f26ec21ff8050d39c3493 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_563dc732738cbe5bdc50b1d326972f78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb079b7bc2ea974b062802c8768cbff2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_786ee15e0ce6d57b70ab7c57983286b7 |
publicationDate | 2018-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108504747-A |
titleOfInvention | A kind of method that calf-derived Cyclospora content in meat products is detected based on digital pcr method |
abstract | The invention discloses a kind of methods detecting calf-derived Cyclospora content in meat products based on digital pcr method.This method application bioinformatics means screen single copy gene sequence from 15 kinds of Animal genome sequences, design internal control primer and ox source species specificity primer.This method carries out absolute quantification analysis based on droplet digital pcr technology to the calf-derived Cyclospora content in meat products, without standard items, without building standard curve, compensate for the defect that part of standards product are not easy to obtain, the magnitude deviation that the process variations between standard items and sample generate also is effectively prevented simultaneously, and then using the percentage composition of reference gene correction ox source detection, obtain genuine and believable food adulteration numerical value.Calf-derived Cyclospora content appraisal can be completed in the detection method of the present invention within 3h, has the characteristics that simple and practicable, efficient and sensible, and mixing pseudo- identification for the quantization of animal sources ingredient opens bright prospects. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113088531-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113088531-B |
priorityDate | 2018-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID962 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419512635 |
Total number of triples: 22.