| abstract |
The present invention relates to the marker of severe asthenospermia and its applications.More specifically, the present invention provides a kind of nucleic acid of the coding ADCY10 mutant of separation, the nucleic acid and SEQ ID NO:1 compares, and has one or both of following mutation：c.2398G>A is mutated, c.3829A>G is mutated, the present invention also provides encode polypeptide, the nucleic acid and the polypeptide of the nucleic acid purposes and detection method in severe asthenospermia detection and thus made of primer and kit.The present invention is using one core families of severe asthenospermia as research object, full sequencing of extron group has been carried out to the diseased individuals in family and has been compared, new Disease-causing gene and mutational site are found, to be detected to severe asthenospermia using the Disease-causing gene and mutational site. |