http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108504618-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P41-001 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P41-00 |
| filingDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108504618-B |
| titleOfInvention | Recombinant bacterium for expressing lipase with R configuration selectivity and application thereof |
| abstract | The invention discloses a recombinant bacterium for expressing lipase with R configuration selectivity and application thereof, wherein the lipase gene with R configuration selectivity is inserted into a multiple cloning site of a pET28a plasmid to obtain a recombinant plasmid; then, the recombinant plasmid is transformed into escherichia coli Shuffle T7 to obtain a recombinant bacterium; the lipase gene selective for the R configuration is the DNA sequence of the following 1) or 2): 1) a DNA sequence shown as SEQ ID NO.2 in the sequence table; 2) a DNA sequence which has more than 90 percent of homology with the DNA sequence limited by 1) and codes the protein with ester hydrolase activity. The recombinant lipase has higher R-configuration stereoselectivity and catalytic activity, the enzyme activity retention is more than 85% under the higher temperature condition, and the recombinant lipase can be used for resolving racemic 2-aryl methyl propionate substances to obtain optically pure S-type 2-aryl methyl propionate substances. |
| priorityDate | 2018-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 335.