| abstract |
The invention discloses a nitrilase mutant and its application. The mutant is the 201st amino acid sequence of the amino acid sequence shown in SEQ ID No. 2, or the 324-381st amino acid sequence by one or more obtained by bit substitution. In the present invention, through protein molecular transformation, the thermostability of the pure nitrilase LNIT5 enzyme at 45°C is increased by 4.5 times at most, and the recombinant Escherichia coli containing the nitrilase mutant is used to hydrolyze 1-cyanide Cyclohexyl acetonitrile, product yield has been improved. Therefore, the mutant obtained in the present invention has a good application prospect in efficiently catalyzing 1-cyanocyclohexylacetonitrile to synthesize gabapentin intermediate 1-cyanocyclohexylacetic acid. |