http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108486069-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-20051 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01F1-0054 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01F41-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01F41-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01F1-00 |
| filingDate | 2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108486069-B |
| titleOfInvention | Virus separation method for low-content sample of porcine epidemic diarrhea virus |
| abstract | The invention discloses a virus separation method for a low-content sample of Porcine Epidemic Diarrhea Virus (PEDV), and also discloses an immune nano magnetic bead for enrichment and separation of porcine epidemic diarrhea virus, wherein the immune nano magnetic bead has magnetic Fe 2 O 3 A core and a surface conjugated with a single domain antibody specific for a PEDV membrane protein. The traditional method for separating virus from multiple infection and low PEDV content samples needs virus purification and long-time blind transmission, has low separation efficiency, and limits research work of PEDV. According to the invention, the immune nano magnetic beads are prepared by using the nano magnetic beads and the PEDV specific single domain antibody, so that PEDV in various samples such as feces and tissues can be effectively captured and enriched. After the sample is processed, the immune nano magnetic beads are used for capturing and enriching viruses, and the immune nano magnetic beads combined with the viruses are directly inoculated to Vero cells, so that the virus separation can be realized. The method for separating the immune nano magnetic beads and the PEDV is suitable for quickly and effectively separating the PEDV in clinically complex samples with low virus content. |
| priorityDate | 2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.