http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108484769-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70514 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-10 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2812 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56966 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
filingDate | 2018-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108484769-B |
titleOfInvention | Monoclonal antibody of anti-paralichthys olivaceus T cell surface marker molecule CD4-2, and preparation method and application thereof |
abstract | The invention disclosesA monoclonal antibody of anti-paralichthys olivaceus T cell surface marker molecule CD4-2 is prepared by the following components: the hybridoma JF-CD4-2-1D8 with the preservation number as follows: CCTCC NO: c201880, the collection unit is: china center for type culture Collection, Address: the preservation date of Wuhan university in Wuhan city of Hubei province is as follows: hybridoma cell secretion on 29/03/2018. The result of indirect enzyme-linked immunoreaction experiments of the monoclonal antibody shows that the monoclonal antibody can be specifically combined with CD4-2 polypeptide, and the result of immunoblotting shows that: the monoclonal antibody can specifically recognize 25kDa paralichthys olivaceus CD4-2 recombinant protein and 33kDa paralichthys olivaceus natural CD4-2 protein, and immune indirect fluorescence and flow cytometry prove that the monoclonal antibody can specifically recognize surface CD4-2 molecules of a paralichthys olivaceus lymphocyte. The invention prepares the monoclonal antibody of anti-paralichthys olivaceus T cell surface marker molecule CD4-2, and aims to detect the paralichthys olivaceus CD4-2 + T lymphocytes provide an important tool, and have important practical significance for deeply understanding the cellular immune mechanism of fishes and developing high-efficiency and green vaccines for fishes. |
priorityDate | 2018-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.