http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108484751-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-765 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-531 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-765 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 |
filingDate | 2018-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108484751-B |
titleOfInvention | Nobiletin antigen and preparation method and application thereof |
abstract | The invention discloses a nobiletin antigen and a preparation method and application thereof, wherein the preparation method comprises the following steps: reacting nobiletin with a compound B containing both amino and carboxyl to obtain a compound C; carrying out coupling reaction on the compound C and N-hydroxysuccinimide in the presence of dicyclohexylcarbodiimide or 1-ethyl- (3-dimethylaminopropyl) carbodiimide hydrochloride to obtain a compound D; performing coupling reaction on the compound D and carrier protein to obtain a nobiletin antigen; the application of the nobiletin antigen in preparing an enzyme linked immunosorbent assay kit, a luminous immunoassay kit or an immunoaffinity chromatographic column for detecting the nobiletin in a sample. The invention has the advantages that: the method can conveniently and quickly obtain the nobiletin antigen, and has the advantages of concise and clear synthesis steps, low synthesis cost and good effect. The antibody obtained by immunizing the nobiletin antigen has good specificity and low minimum detection limit. |
priorityDate | 2018-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.