| abstract |
The present invention provides culture platforms, cell culture media and methods for differentiating pluripotent cells into hematopoietic cells. The present invention further provides pluripotent stem cell-derived hematopoietic cells produced using the culture platforms and methods disclosed herein that allow feeder-free monolayer culture without EB formation. Specifically, the pluripotent stem cell-derived hematopoietic cells of the present invention include but are not limited to iHSCs, permanent hematopoietic endothelial cells, hematopoietic multipotential progenitor cells, T cell progenitor cells, NK cell progenitor cells, T cells, NK cells, NKT cells and B cells. |