http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108473946-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12622c5504b328fa3843e22c6f4b3f01 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2330-51 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2016-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f15e5a7b15df8e7db9fb80a0b2ebe3b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b64b1716967ddb4d6112444d84b12f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_875111d2993ca4a9e9fb035e799d3e78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6824f190831df35af142e6b19c0cfa1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1bc1bfc5349e95099a13938db7d8d6e |
| publicationDate | 2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108473946-A |
| titleOfInvention | Enhanced protein expression and methods thereof |
| abstract | The present disclosure generally relates to modified Gram-positive bacterial cells that produce increased amounts of one or more proteins of interest and modified Gram-positive bacterial cells that have enhanced genetic competence. Accordingly, certain embodiments of the present disclosure relate to a modified Gram-positive bacterial cell expressing an increased amount of the same protein of interest relative to an unmodified (parental) Gram-positive bacterial cell expressing the same protein of interest, wherein the The modified bacterial cell comprises at least one mutation in the rpoC gene encoding a variant RNA polymerase (RNAP) β'-subunit polypeptide. In certain embodiments, said rpoC gene encoding a variant β'-subunit polypeptide is integrated into the chromosome of said modified cell. In other embodiments, said rpoC gene encoding a variant β'-subunit polypeptide is comprised in an extrachromosomal plasmid introduced into said modified cell. In other embodiments, the present disclosure relates to a competent Bacillus host cell comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide, the modified rpoC polypeptide Comprising 90% sequence identity to SEQ ID NO: 8 and a substitution from aspartic acid to glycine at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is essential for introducing the The first nucleic acid construct is foreign to the previously non-competent Bacillus host cell. |
| priorityDate | 2015-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 721.