| abstract |
The present invention relates to a kind of method detecting dopamine based on nitrogen-doped graphene quantum dot fluorescence quenching mechanism, step is：（a）Nitrogen-doped graphene quantum dot is prepared,（b）The fluorescence spectrum of standard solution is drawn,（c）Testing sample fluorescence intensity is measured, dopamine concentration is calculated.The present invention can reduce the fluorescence intensity of nitrogen-doped graphene quantum dot using the dopamine of various concentration, dopamine concentration is in bilinear relation with fluorescent quenching intensity, it can detect that the dopamine concentration of wide concentration range, 4000 nM of linear ranging from 0.05 nM of detection, it measures quick and easy, interference is few, can be used for measuring the DOPAMINE CONTENT IN RABBIT in blood, drug. |