http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108469427-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-44 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-44 |
| filingDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108469427-B |
| titleOfInvention | Method for representing plasma-induced intracellular absolute calcium ion concentration |
| abstract | The invention relates to a method for representing plasma-induced intracellular absolute calcium ion concentration. The method adds the prepared cell suspension into the optimized Fluo-3AM, and adds the cell suspension into the cell suspension by 0.8mM Pluronic F-127, maximum fluorescence intensity F max Value, or addition of the calcium chelating agent EGTA, to obtain the minimum fluorescence intensity F min And (3) adding optimized Fluo-3AM into the prepared cell suspension, treating the cell suspension by atmospheric pressure cold plasma, measuring an F value by combining a multifunctional microplate reader, and calculating the absolute calcium ion concentration according to a formula. The method for measuring the absolute calcium ion concentration of the cells by combining the fluorescent probe Fluo-3AM with the multifunctional microplate reader is realized, and the method has the advantages of sensitivity, high efficiency, simplicity, convenience and accuracy in measuring and representing the absolute calcium ion concentration in the cells. |
| priorityDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.