http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108467847-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
| filingDate | 2018-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108467847-B |
| titleOfInvention | Recombinant bacillus subtilis capable of extracellularly secreting inulinase and preparation method and application thereof |
| abstract | The invention discloses a recombinant plasmid, which is obtained by overlapping a CDS sequence inuQ of inulinase and a signal peptide by using overlapping PCR (polymerase chain reaction), and then connecting an obtained fragment containing the signal peptide and a large intestine-hay-bacillus shuttle plasmid pPsacB-NMK together. The invention also discloses a bacillus subtilis transformed by the recombinant plasmid WB800‑R The obtained recombinant bacillus subtilis can secrete inulinase extracellularly. The invention also discloses a preparation method of the recombinant plasmid and the recombinant bacillus subtilis, and an application of the recombinant bacillus subtilis. The recombinant bacillus subtilis does not have the potential safety hazard of producing fructo-oligosaccharide by escherichia coli, has higher advantage than other engineering bacteria in the aspect of producing inulin endonuclease, and can obtain DP 3 ，DP 4 ，DP 5 The fructo-oligosaccharide with polymerization degree can be prepared by a one-step fermentation method, so that the time is saved, and the strict conditions of enzyme catalysis are reduced. |
| priorityDate | 2018-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 116.