| abstract |
The present disclosure generally relates to the use of mechanical and/or biochemical dissociation methods to homogenize intact samples, or substantial portions thereof, from clinical samples such as tumors (in whole or in part), lymph nodes, metastases, cysts, polyps, or Combinations or parts thereof to prepare representative samples. Despite spatial heterogeneity within this sample, the resulting homogeneity provides the ability to obtain a correctly representative sample, increases the likelihood of detection of low prevalence subclones, and is suitable for a variety of diagnostic assays as well as for the production of therapeutics, especially "Personalized" anti-tumor vaccines or immune cell-based therapies. |