http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108441547-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2018-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108441547-B |
titleOfInvention | Primer group, kit and method for HLA gene amplification and genotyping |
abstract | The invention provides a primer group, a kit and a method for HLA gene amplification and genotyping, belonging to the field of gene detection. The primer group for HLA gene amplification is characterized in that primers are designed according to 8 exon regions of HLA-A genes, 8 exon regions of HLA-B genes and exons 2 and 3 of HLA-DRB1 genes respectively, and PCR amplification is carried out on the HLA genes by using the primers to obtain a product with the gene fragment length of more than 400bp and less than 1.5 kb; the HLA gene is amplified by the primer PCR, so that the length of a gene fragment of an amplification product is more than 400bp and less than 1.5kb, the requirement on the integrity of a template is reduced, the amplification efficiency is high, the amplification reaction time is short, the cost is reduced, and the requirement on the amplification or genotyping of the HLA gene of a large-scale sample can be met. |
priorityDate | 2018-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.