abstract |
The invention discloses a method for promoting the replication of bovine rhinotracheitis virus, which comprises the steps of irradiating bovine kidney cell culture medium with a low-temperature atmospheric pressure plasma generator, adding the irradiated medium into bovine kidney cells, and adding bovine nose Tracheitis virus incubation. The invention indirectly treats the cells through the plasma, and the treatment is uniform and the intensity is controllable. The processing time of the present invention only needs 2 minutes, and the operation is simple and fast. The treated DMEM medium is incubated with bovine rhinotracheitis virus and bovine kidney cells for 1 hour, which can significantly improve the replication of bovine rhinotracheitis virus in bovine kidney cells, and the obtained high concentration The bovine rhinotracheitis virus can be used in vaccine production after inactivation treatment, and the vaccine production efficiency can be significantly improved. |