http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108424900-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-05001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-002 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 |
| filingDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108424900-B |
| titleOfInvention | A kind of nitrilase mutant and its construction method and application |
| abstract | The invention discloses a nitrilase mutant, a construction method and an application in the synthesis of a pregabalin chiral intermediate, and belongs to the technical field of bioengineering. In the present invention, the turnip nitrilase BrNIT and the Arabidopsis nitrilase AtNIT are respectively used as parents, and the peptide segment at the 225-285 position of the alpine Arabidopsis nitrilase AaNIT is used to replace the 226-286 position of the amino acid sequence of BrNIT. and position 225-285 of the amino acid sequence of AtNIT to obtain nitrilase mutants BrNIT 225-285 and AtNIT 225-285 whose amino acid sequences are shown in SEQ ID NO.1 or SEQ ID NO.3. Compared with the wild-type nitrilase, the nitrilase mutant provided by the present invention significantly improves the activity of catalyzing the hydrolysis of racemic isobutylsuccinonitrile and the stereoselectivity of the product, which can meet the requirements of industrial application, and at the same time, it can effectively catalyze the hydrolysis of racemic isobutyl succinonitrile. The racemic isobutyl succinonitrile has a good application prospect in the synthesis of 3-cyano-5-methylhexanoic acid. |
| priorityDate | 2018-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 439.