http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108410966-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108410966-B |
| titleOfInvention | Method for detecting insertion and deletion of ACE gene |
| abstract | The invention discloses a method for detecting insertion and deletion of an ACE gene, which comprises the following steps: step S10, adding a probe with HEX mark and 1 pair of primers into a sample to be tested for reagent preparation; step S20, carrying out allelic PCR amplification on the reagent; in step S30, a dissolution curve is performed by means of the LC480 platform and the corresponding fluorescence signal is collected. The invention has the advantages that mature fluorescent quantitative PCR technology is utilized, only 1 HEX-labeled probe and 1 pair of common primers are needed, common unequal PCR is firstly carried out, then melting curves are operated on various fluorescent quantitative PCR instruments, and 3 genotypes of ACE gene polymorphism II, ID and DD can be completed in about 90 minutes. The method has the advantages of simple operation, rapid detection, clear and accurate interpretation, low cost, wide application in various instruments, high flux and the like. |
| priorityDate | 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.