http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108384889-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108384889-B |
| titleOfInvention | Multiple RT-PCR kit for genotyping and identifying bluetongue virus and detection method thereof |
| abstract | The invention discloses a multiple RT-PCR kit for typing and identifying BTV-2, 3, 4, 7 and 12 genotypes and a detection method thereof, which are used for simultaneously identifying and detecting the bluetongue virus 2, 3, 4, 7 and 12 in a single tube. The method designs 5 pairs of PCR specific primers according to conserved regions of VP2 gene sequences of different genotypes of bluetongue virus, synthesizes a pair of universal primers of non-biological sources by utilizing the GeXP principle, respectively adds the universal primers to the upstream and downstream of each pair of the specific primers to form 5 pairs of specific chimeric primers, carries out reverse transcription by using the specific primers, and combines the universal primers and the specific chimeric primers to construct the multiplex PCR. By utilizing an optimized multiplex PCR system and conditions, one or more of 5 genotypes such as BTV type 2, type 3, type 4, type 7, type 12 and the like can be identified and detected simultaneously by a single tube, the nucleic acid of other types of BTV, PPRV and FMDV is amplified without specificity, and the lowest detection concentration can reach pg level. The method established by the invention has the advantages of high sensitivity, strong specificity, time and labor saving and easy observation of results. |
| priorityDate | 2018-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.