| abstract |
The invention discloses a kind of methods of the high enzyme activity amylase of expression, first knock out the AodevR genes in aspergillus oryzae, the expression of amylase is then carried out using the bacterial strain, obtains high enzyme activity amylase.The present invention is replaced the AodevR genes in aspergillus oryzae with selectable marker gene pyrG by homologous recombination, and to obtain AodevR knock-out bacterial strains, the expression of amylase is then carried out using the bacterial strain, can obtain high enzyme activity amylase, and test result confirms：The missing of AodevR significantly improves the activity of Amylase EC, and the 2nd day, the amylase activity of AodevR knock-out bacterial strains was 3 times of reference strain, and in the 5th day increase to reference strain 7 times. |