| abstract |
The present invention relates to field of gene, more particularly to a kind of slow virus carrier and application of sgRNA and its building, the nucleotide sequence of the sgRNA is as shown in SEQ ID NO.1-3, present invention employs most efficient CRISPR/Cas9 gene editing tools at present, the designed site gene sgRNA HIF-1 α has the gene knockout activity better than other sites of previous research institute report, joint TAE/TACE operation for the first time, is used for HCC oncotherapy. |