http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108330183-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_724a7439a7bcbcd1a2066efa3e43407e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2018-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0c9c9b449ec2fd1a73261afa388ddab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98f2809231a0e9822f632e6df208fc5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dba255d7ca20294b29b41a516d46ecd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03e78fed37576a140112b4386c5ae323 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5acf920cdeab574c4f772089bc0d3526 |
| publicationDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108330183-A |
| titleOfInvention | A kind of qRT-PCR detection method of plasma miRNA |
| abstract | The invention discloses a qRT-PCR detection method for plasma miRNA, comprising the following steps: collecting specimens, adding miRNA extract solution dropwise, adding chloroform dropwise, centrifuging for the first time, pipetting, adding miRNA extract solution and isopropanol dropwise, Second centrifugation, dropwise addition of ethanol, third centrifugation, dropwise addition of RNase‑free water, configuration of tailing and reverse transcription reaction system, configuration of fluorescent quantitative PCR reaction system, quantitative detection, reading and recording of data and data analysis; The present invention can detect various parameters of miRNA in blood plasma. At present, the specific mechanism of miRNA participating in these complex gene regulation and conduction pathways of various diseases is not clear, so the target gene of miRNA can be accurately predicted and the target gene can be accurately predicted. Systematic biological analysis of conduction pathways is also an important link in the study of their mechanisms of action. Bioinformatics analysis can effectively predict miRNA target genes and construct their regulatory networks, providing a theoretical basis for miRNAs in the diagnosis of diseases. |
| priorityDate | 2018-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.