http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108330132-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-325 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
| filingDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108330132-B |
| titleOfInvention | A kind of artificially synthesized cry1Ah1 gene and its application |
| abstract | The invention discloses an artificially synthesized cry1Ah1 gene and its application. The base sequence of the artificially synthesized cry1Ah1 gene is shown in SEQ ID NO.2 or as shown in SEQ ID NO.3. In the present invention, the wild-type sequence of the Bt insecticidal gene crylAh1 gene is selected as the original sequence, and the full-length start codon of the cry1Ah1 gene coding region starts from the 64nt-1998nt insecticidal key region with a total of 645 amino acids, without changing the original amino acid primary sequence. Codon optimization was carried out under the premise to obtain cry1Ah1-B gene and cry1Ah1-U gene, and the proteins translated by all genes of the optimized gene and the pre-optimized gene were completely consistent through tBlastX comparison. The RT-PCR identification and analysis of the transgenic cry1Ah1 transgenic plants showed that the expression efficiency of cry1Ah1-B, cry1Ah1-U and the wild-type cry1Ah1 gene was significantly different. The total protein content of the line was maintained between 24.013mg/g and 26.667mg/g, and A-3-4, A-5-0, A-5-23 and Z-1-3 were among the lines with relatively good insect test results. The toxic protein content of the four strains was between 4669.38ng/g and 9342.33ng/g, and the anti-insect effect was obvious. |
| priorityDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.