http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108330095-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01056 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y504-02003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 |
| filingDate | 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108330095-B |
| titleOfInvention | Recombinant corynebacterium glutamicum for accumulating N-acetylneuraminic acid and application thereof |
| abstract | The invention discloses a recombinant corynebacterium glutamicum for accumulating N-acetylneuraminic acid and application thereof, belonging to the field of genetic engineering. The invention takes corynebacterium glutamicum as an expression host, and strengthens the synthesis path of N-acetylneuraminic acid by over-expressing glucosamine-fructose-6-phosphate aminotransferase gene, glucosamine acetylase coding gene, phosphatase coding gene, acetylglucosamine isomerase coding gene and N-acetylneuraminic acid synthase coding gene; by knocking out the coding gene of N-acetylneuraminic acid transport protein in the corynebacterium glutamicum and the related gene on the intracellular N-acetylneuraminic acid decomposition and utilization metabolic pathway, the corynebacterium glutamicum genetic engineering strain with extracellular accumulation of N-acetylneuraminic acid is obtained, the yield reaches 110mg/L, and a foundation is laid for further metabolic engineering modification of corynebacterium glutamicum to produce N-acetylneuraminic acid. |
| priorityDate | 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1089.