http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108315298-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0636 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0783 |
| filingDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108315298-B |
| titleOfInvention | Gamma T cell culture method |
| abstract | The invention provides a culture method of gamma T cells, which comprises the following steps: (1) obtaining mononuclear cells from peripheral blood, carrying out heavy suspension by using serum-free culture medium additionally added with antibody, adjusting the density of the mononuclear cells, and culturing in an incubator; (2) on day 4 and day 8, serum-free culture media without the addition of antibodies were supplemented respectively; on day 12, the amplification culture is finished; (3) screening the T cells, transferring the sorted gamma T cells to a cell culture bottle, and supplementing a gamma T cell culture medium; (4) on day 4 and day 8, the gamma T cell culture media were supplemented, respectively; on day 12, the amplification culture is finished; (5) the gamma T cells were collected. The invention has the advantages that: provides a cell culture method specially aiming at gamma T cells, the gamma T cells are amplified for 12 days, the amplification multiple reaches 60 times, and the killing activity of the cultured gamma T cells on K562 tumor cells reaches more than 90 percent when the ratio is 25: 1. |
| priorityDate | 2018-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.