http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108300791-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108300791-B |
| titleOfInvention | Synchronous detection and identification method for three main pathogenic nematodes in tobacco root knot nematode disease |
| abstract | The invention discloses a synchronous detection and identification method for three main pathogenic nematodes in tobacco root knot nematode disease. Comprises the steps of PCR primer design, DNA template preparation, PCR amplification and product detection. The invention designs the PCR primers containing 6 for identifying the species of the 3 kinds of root-knot nematodes according to the species specific region sequences of the meloidogyne incognita, the meloidogyne arachidis and the meloidogyne javanica, and the identification stability and the accuracy are good; the designed 6 primers are combined for use, so that 1 time of PCR reaction can be realized to complete the synchronous detection of the pathogenic nematodes of the 3 tobacco root-knot nematode diseases, and the identification is convenient and time-saving; a common PCR instrument and a conventional reagent are adopted, so that the detection cost is low, and the popularization and the application are facilitated; the invention comprises a DNA preparation method aiming at diseased root samples and soil samples, can identify the species of root-knot nematodes in various development stages including eggs, larvae and adults, and has wide application range. |
| priorityDate | 2018-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 639.