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filingDate 2018-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf6376a9f8d792eaf5338319d19288d4
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publicationDate 2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108300785-A
titleOfInvention A kind of primer probe combination and application thereof for BRAF gene mutation detection
abstract The present invention relates to a gene mutation detection product, in particular to a primer probe combination for BRAF gene mutation detection and its application. The primer probe combination includes a detection primer probe, and the detection primer probe includes BRAF gene V600E Mutation detection specific primer pair, BRAF gene specific probe, amplification blocking probe and internal reference system; wherein, the mismatch rate of the BRAF gene V600E mutation detection specific primer pair is 15-30%. The invention has the advantages of strong specificity, high sensitivity, simple and fast operation for BRAF gene mutation, good accuracy and repeatability of detection results, can detect tumor tissue samples, can assist clinical treatment, and has important value.
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