http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108300759-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
| filingDate | 2018-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108300759-B |
| titleOfInvention | Method for detecting PARP-1 activity based on fluorescent dye TOTO-1 analysis |
| abstract | The invention discloses a method for detecting PARP-1 activity based on TOTO-1 fluorescent dye analysis. The method comprises the following steps: (1) activating DNA, PARP-1 (poly (adenosine diphosphate ribose) polymerase-1), NAD + (nicotinamide adenine dinucleotide) mixed reaction, PARP-1 catalyzes the synthesis of PAR polymer (poly ADP-ribose) with a large amount of negative charge; (2) the resulting product was double-stranded with ExoIII. Release of PAR; (3) TOTO-1 (thiazole orange dimer-1) was reacted with the product PAR polymer and the product solution was examined by fluorescence spectroscopy. The invention utilizes the enhancement effect of the fluorescence signal generated after TOTO-1 is combined with the PAR polymer product to observe the change of the fluorescence signal intensity, and can be used for detecting PARP-1 enzyme. The invention has the advantages of simplicity, convenience, rapidness, high sensitivity and no need of marking a DNA probe. |
| priorityDate | 2018-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.