http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108285906-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-108 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108285906-B |
| titleOfInvention | Construction method of site-specific integration exogenous DNA transgenic pig |
| abstract | The invention discloses a construction method of a site-specific integration exogenous DNA transgenic pig, which comprises the following steps: s1, screening safety targets and verifying the cutting efficiency of target binding gRNA; s2, constructing a homologous arm donor plasmid, and obtaining a site-directed integration transgenic cell line; s3, constructing the exogenous DNA site-directed integration transgenic pig. The gRNA target sequence is introduced into the donor plasmid, the gRNA transcribed in the cell is utilized to induce the Cas9 nuclease to cut the target gene, and simultaneously, the donor plasmid is linearized, so that the test steps are greatly simplified, the labor is saved, and the cotransfection efficiency is favorably improved. The invention uses fewer vectors for site-specific integration, has moderate homologous arms, is more favorable for obtaining a transgenic cell line, combines a high-efficiency site-specific integration technology, a high-activity site-specific transgenic cell culture technology and a somatic cell cloning technology, is favorable for efficiently preparing site-specific integrated transgenic animals, and accelerates the culture speed of new varieties of transgenic animals. |
| priorityDate | 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 349.