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filingDate 2017-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108279278-B
titleOfInvention Method for separating flavonoid components and application thereof
abstract The invention relates to a method for separating flavonoid components with similar structures, which is based on high performance liquid chromatography, wherein the flavonoid components with similar structures at least comprise astragalin and quercitrin; the chromatographic conditions are as follows: stationary phase: octadecylsilane chemically bonded silica; mobile phase: the mobile phase A is acetonitrile, the mobile phase B is 0.05-0.5% (volume percentage) of phosphoric acid aqueous solution, and gradient elution is adopted: 0-40min, 7-25% of A and the balance of B; 40-60min, 25-50% of A and the balance of B. The method realizes the separation of astragalin and quercitrin for the first time. The invention also provides application of the method in detection of flavonoid components in the persimmon leaf extract, and particularly relates to application in determination of the content of the flavonoid components in the persimmon leaf extract or a preparation thereof based on a one-test-multiple evaluation method.
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