| abstract |
The present invention provides the detection architecture and its detection method of a kind of nucleic acid and application, a diameter of 0.5 2 μm, the individual layer molybdenum disulfide nano sheet that thickness is 0.5 2nm of exonuclease III of the detection architecture including 30 50U, fluorescence probe and 60 80 μ g/mL；The present invention is by the way that individual layer molybdenum disulfide nano sheet is applied in the fluorescent detection system of exonuclease III, for specific nucleotide sequence, optimize reaction condition and flow, function is quenched in the cycle enlarging function and molybdenum disulfide nano sheet for utmostly playing exonuclease III, each step is synergistic, improves the sensitivity and specificity of detection architecture, easy to operate, it is succinct efficient, it has broad application prospects. |