| abstract |
The present invention provides one kind for Respiratory Syncytial Virus(RSV) nucleic acid parting detecting reagent, kit includes RT PCR reaction solutions, RT PCR enzymatic mixtures, Respiratory Syncytial Virus(RSV) serotype specific primer probe, internal reference, negative control, critical positive control and robust positive control.The present invention can directly carry out the Respiratory Syncytial Virus(RSV) nucleic acid extracted one-step method RT PCR reactions, carry out qualitative parting detection to the A types in sample and Type B Respiratory Syncytial Virus(RSV), and rely on reference gene as internal reference, utilize UNG enzyme Pollution protections.The kit one step amplification method of the present invention is simple, program is brief, easy to operate, Pollution protection, testing result high specificity, susceptibility are high, result is apparent, with a high credibility, available for the qualitative Classification Identification of Respiratory Syncytial Virus(RSV), detection in people's Nasopharyngeal swabs sample. |