http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108239652-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24122 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18 |
filingDate | 2016-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108239652-B |
titleOfInvention | Zika virus capsid protein rigid fusion expression vector and construction and application thereof |
abstract | The invention discloses a Zika virus capsid protein rigid fusion expression vector, construction and application thereof, wherein the nucleotide sequence of MBP-ZIKV _ capcid in the Zika virus capsid protein rigid fusion expression vector MBP-ZIKV _ capcid _ pET22b is shown by SEQ ID NO. 1. The Zika virus capsid protein can be massively expressed and purified in vitro by utilizing rigid fusion of the MBP gene and the Zika virus capsid protein gene, and after the Zika virus capsid protein rigid fusion expression vector is transformed into a host cell, the problems of low protein expression level and high protein aggregation of single Zika virus capsid protein in the prior art are solved, the protein solubility is improved, the protein is easy to purify, the Zika virus capsid protein can be efficiently obtained, a new technology is provided for purifying the protein and related proteins thereof, and the method can be used for development of related products of medical detection and biological function experiments. |
priorityDate | 2016-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.