| abstract |
The invention discloses a composite nanomaterial based on carbon dot-heme imitating peroxidase, through which a visible and fluorescent dual-channel visual detection method for hydrogen peroxide, glucose and xanthine is established. This method catalyzes the oxidation of phenol and 4-aminoantipyrine with hydrogen peroxide by carbon dot-heme peroxide mimetic enzyme to form a stable red color product, and the maximum absorption peak of the color product is 505 nm; at the same time, The fluorescence of the reaction solution at the excitation wavelength of 480 nm and the emission spectrum of the carbon dot-heme nanomaterial is quenched at 540 nm. Therefore, the absorbance of the chromogenic product at 505 nm and the fluorescence quenching signal at the emission spectrum of 540 nm are different from hydrogen peroxide, glucose and xanthine at 1.7×10 ‑7 ‑2×10 ‑4 M, 1.7×10 ‑7 ‑1.3×10 ‑4 M and 3.3×10 ‑6 ‑3.3×10 ‑5 M ranges are linear. |