http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108203714-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108203714-B |
| titleOfInvention | Cotton gene editing method |
| abstract | The invention belongs to the technical field of plant genetic engineering, and particularly relates to a cotton gene editing method. A CRISPR-Cas9 system for cotton genome editing and a construction method thereof are disclosed. The invention clones two upland cotton U6 promoters pGhU6-7 and pGhU6-9, and mutates Bsa I restriction enzyme sites. Two vectors of the CRISPR-Cas9 vector pRGEB32-GhU6.7-NPT II and pRGEB32-GhU6.9-NPT II which have editing capacity in cotton are constructed by using the optimized promoter. Selecting 1 cotton endogenous gene GhPLA as a target gene, designing four sgRNAs targeting the gene, and guiding Cas9 protein to cut at a specific site of the GhPLA. The albino phenotype, the enzyme cutting map and the sequencing verification of cotton show that pGhU6-7I and pGhU6-9I driven CRISPR-Cas9 gene editing system has editing capacity in cotton. |
| priorityDate | 2016-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.