abstract |
The invention discloses a kind of specific molecular marker of Scatophagus argus (Linnaeus) genetic sex, DNA fragmentation of the molecular labeling for two homeologous in Scatophagus argus (Linnaeus) X chromosome and Y chromosome, DNA fragmentation nucleotide sequence such as SEQ ID NO on X chromosome:Shown in 1, DNA fragmentation nucleotide sequence such as SEQ ID NO on Y chromosome:Shown in 2.The present invention screens two part homologous DNA fragments on Scatophagus argus (Linnaeus) X chromosome and Y chromosome from Scatophagus argus (Linnaeus) genomic information for the first time, and designs specific primer and carry out Scatophagus argus (Linnaeus) genetic sex identification, can fast and accurately distinguish Scatophagus argus (Linnaeus) genetic sex.The method of the present invention is suitable for laboratory or aquaculture enterprise precise Identification Scatophagus argus (Linnaeus) genetic sex.Present invention detection Scatophagus argus (Linnaeus) heredity method for distinguishing can be used for the Scatophagus argus (Linnaeus) sex identification to different stages of growth and different groups and screening, to research Scatophagus argus (Linnaeus) Sex Determination and differentiation mechanism, realize that its sexual control is of great significance. |